BIOTRANSFORMATION OF CARAWAY OIL BY LACCASE PRODUCED FROM Pleurotussapidus

Abstract

In this study, laccase produced and purified from Pleurotus sapiduswas studied. Production of laccase induced by four type of inducer (CuSO_4, ABTS, Xylidin, and ABTS+CuSO₄) as inducer. The maximum laccase activity observed while P. sapidusin the presence of xylidin as inducer (1.5 U/ml). Laccase purification was performed by precipitated the enzyme with 90% ammonium sulphate followed by gel filtration with sephadex G25. The optimum laccase activity was observed at acidic pH values (close to 4.6- 5.6), while the optimum temperature 40 C. The ability of enzyme to bio- transform Caraway oil was detected at different reaction medium. Biotransformation of caraway oil in presence of three mediators (40 mM HBT, 40 mM TEMPO, 10 mM ABTS) was more effective than others systems.